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PerkinElmer LAS |

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Á¦Ç°¸í |
VICTOR3 |
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Ä«´Þ·Î±× ¹øÈ£ |
¹®ÀÇ¿ä¸Á |
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Á¦Ç°´ã´çÀÚ |
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[Á¦Ç°°³¿ä]
º» Á¦Ç°Àº ½Ã·áÀÇ Èí±¤(Absorbance), Çü±¤(Fluorescence), ¹ß±¤(Luminescence), ½Ã°£ºÐÇØÇü±¤(TRF),
Æí±¤(Fluorescence Polarization) µîÀ» ÇÑ ±â±â ³»¿¡¼ ÃøÁ¤ÇÒ ¼ö ÀÖ´Â ´Ù±â´É Microplate ÀåºñÀÔ´Ï´Ù.
1-well ¿¡¼ 1536-well plate, PCR tube, Dot blot membrane ±îÁö ´Ù¾çÇÑ ½Ã·á ¿ë±â¸¦ »ç¿ëÇÒ
¼ö ÀÖ½À´Ï´Ù. ¶ÇÇÑ Dispenser¸¦ ÀåÂøÇÏ¿© Flash luminescenceµµ °¡´ÉÇÏ¸ç °íÈ¿À² È¿´É °Ë»ö (HTS)À» À§ÇÑ
Robot system°úµµ ½±°Ô ¿¬°áÇÒ ¼ö ÀÖ½À´Ï´Ù. Cell based assay¸¦ ºñ·Ô immunoassays, receptor
assays, kinetic assay, Drug screening µî ÀÀ¿ëºÐ¾ß°¡ ´Ù¾çÇÑ Â÷¼¼´ë Bench top ÀåºñÀÔ´Ï´Ù.
[Á¦Ç°ÀÇ Æ¯ÀåÁ¡]
1. ±â´É - Èí±¤ (Absorbance) : 340 - 800 nm, UV Èí±¤ : 260 nm/280 nm
- Çü±¤ (Fluorescence) : 340 - 850 nm
- ¹ß±¤ (Luminescence) : Glow/Flash/Dual ÃøÁ¤
- ½Ã°£ºÐÇØÇü±¤ (TRF)
- Æí±¤ (Fluorescence Polarization) : 400 - 850 nm
2. Kinetic assay °¡´É
°°Àº Ç÷¹ÀÌÆ®¸¦ ¿¬¼ÓÇÏ¿© 99ȸ±îÁö ÀÚµ¿À¸·Î ÃøÁ¤ÇÒ ¼ö ÀÖÀ¸¸ç, °°Àº wellÀ» 100ȸ±îÁö
ÃøÁ¤ ÇÒ¼ö ÀÖ´Ù.
3. Scanning ±â´É
MembraneÀ̳ª Chip, Slide¸¦ »ç¿ëÇÏ¿© Cell based assay ½ÇÇè½Ã À¯¿ëÇϸç,
scanning point¸¦ ÃÖ¼Ò 0.1 mm°£°ÝÀ¸·Î ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù.
4. Dual ratio ÃøÁ¤ °¡´É
ÇϳªÀÇ protocol ¿¡¼ PMTÀÇ calibration ¾øÀÌ µÎ °¡ÁöÀÇ excitation filter¿Í
Emission filter¸¦ °¢°¢ ¼±ÅÃÇÏ¿© ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù. ƯÈ÷ ÀÌ mode´Â ÇϳªÀÇ Çü±¤¹°Áú¿¡¼
 µÎ °¡ÁöÀÇ Excitation µÇ´Â ÆÄÀåÀ» ÃøÁ¤ÇؾßÇÏ´Â Ca2+ ½ÇÇè¿¡ ±²ÀåÈ÷ À¯¿ëÇÏ´Ù.
5. Top and Bottom ÃøÁ¤
Çü±¤ ¸ðµå ÃøÁ¤½Ã »ç¿ëµÇ¸ç, ½Ã·á ¿ë±âÀÇ À§¿¡¼ ÃøÁ¤ÇÏ´Â Top ¸ðµå´Â ÀϹÝÀûÀ¸·Î »ç¿ë
µÇ´Â °¡Àå È¿°úÀûÀÎ ÃøÁ¤ ¸ðµåÀ̸ç, ¶Ñ²±ÀÌ ÀÖ°í ½Ã·á¿ë±â¿¡ °íÂøµÈ ¼¼Æ÷¸¦ ÃøÁ¤ÇØ¾ß ÇÏ´Â
°æ¿ì¿¡ Bottom ¸ðµå¸¦ ¼±ÅÃÇÏ¿© ½Ã·á ¿ë±â ¾Æ·¡¿¡¼ ÃøÁ¤ÇÔÀ¸·Î½á ÃøÁ¤°ªÀ» ±Ø´ëÈÇÒ ¼ö
ÀÖ´Ù.
6. Dual Window TRF
½Ã°£ºÐÇØ Çü±¤ (TRF) ÃøÁ¤½Ã ½Ã·á·ÎºÎÅÍ ¹ß»ýµÇ´Â ¶õź³ªÀ̵å°è¿ÀÇ Çü±¤¹°ÁúÀÇ ¿¡³ÊÁö
¾çÀ» Á¤È®ÇÏ°Ô ÃøÁ¤Çϴµ¥ À¯¿ëÇÏ´Ù.
7. ¿¡³ÊÁö¿ø
Tungsten-Halogen Lamp : 340 -800 nm
UV Xenon Flash Lamp: 260 mn / 280 nm / 340 nm
8. Filter »ç¿ë: »ç¿ëÀÚÀÇ ½ÇÇè¿¡ µû¶ó °¡Àå Á¤È®ÇÑ filter¸¦ ¼±ÅÃÇÏ¿© ÀåÂøÇÒ ¼ö ÀÖ´Ù.
Excitation filter: Á÷°æ 15 mmÀÇ 8°³, 25.4 mmÀÇ 4°³
Emission filter: Á÷°æ 25.4 mmÀÇ 16°³
9. Detection Unit
PMT (Photomultilplier tube): Çü±¤, ¹ß±¤, TRF ÃøÁ¤ ¸ðµå
Photodiode: Èí±¤ ¸ðµå
10. Focusing
½Ã·á¿ë±â·ÎºÎÅÍ ½Ã·á¸¦ °¨ÁöÇÏ´Â Detector±îÁöÀÇ ÃÊÁ¡À» 3 - 18 mm·Î Á¶Àý°¡´ÉÇϸç,
½Ã·áÀÇ volumeÀ» °¨¼ÒÇØ¾ßÇÏ´Â ½ÇÇè½Ã S/N ºñÀ²À» ³ô¿© ÃøÁ¤°ªÀ» ÃÖÀûÈÇÒ ¼ö ÀÖ´Ù.
11. Shaker
linear, orbital, double orbital µî ¼¼°¡Áö ¸ðµå¿Í ¼Óµµ ¹× ½Ã°£À» Á¶ÀýÇÏ¿©
½Ã·á¸¦ ÃÖÀûÀÇ Á¶°ÇÀ¸·Î È¥ÇÕÇÒ ¼ö ÀÖ´Ù.
12. ½Ã·á ¿ë±â
¸ðµç typeÀÇ 1 -1536 well plate, Petri dish, slide, filter, Terasaki plate,
PCR plate µî ´Ù¾çÇÑ ½Ã·á ¿ë±â¸¦ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
[Application]
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Cell Based Assay
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Cytotoxicity, Cell Viability, Cell Quantification, Cell Proliferation, Cell adhension, NK cell activity, Oxidiatve burst, Calcium uptake, Reporter gene, Phogocytosis
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Binding studies
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Immunoassays, reporter assays, SNP detection
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Environmental toxins, Food monitoring
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Kinetic Assay
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Enzyme activity, Ca2+ mesurements
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Molecular Biology
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Hybridization, DNA quantification, Gene expression, PCR product quantification
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Quantifications
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Protein, ssDNA, cells, bacteria, cytokines, Cholesterol, Fatty acid, ATP
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Toxicology
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| Drug screening |
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